| 產(chǎn)品名稱 | PWR-1E |
|---|---|
| 商品貨號(hào) | B165540 |
| Organism | Homo sapiens, human |
| Tissue | prostate |
| Cell Type | epithelial |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain Adenovirus 12 and SV40 DNA viral sequences] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | 67 years |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype | At passage 42, a majority of the cells were in the 56-59 range; XY
Ref  Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420 |
| Derivation | Tumor Suppressor gene(s):p53 + [PubMed: 8761420] Human prostatic epithelial cells, derived from a normal prostate with mild hyperplasia, were immortalized with an adenovirus 12-SV40 hybrid virus (Ad12-SV40).
PWR-1E cell line (ATCC CRL-11611) was derived by single cell cloning of a non-producer cell [PubMed: 8761420].
|
| Clinical Data | 67 years
male |
| Antigen Expression | kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen) |
| Receptor Expression | androgen receptor, expressed Ref Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420(upregulated upon exposure to androgen) |
| Genes Expressed | cytokeratin 18,cytokeratin 8,SV40 large T antigen,kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen) |
| Cellular Products | cytokeratin 18 cytokeratin 8 SV40 large T antigen |
| Tumorigenic | No |
| Effects | No, in nude mice (with or without Matrigel) Yes, the cells do form colonies in soft agar |
| Comments | Tumor Suppressor gene(s): p53 + [PubMed: 8761420] Human prostatic epithelial cells, derived from a normal prostate with mild hyperplasia, were immortalized with an adenovirus 12-SV40 hybrid virus (Ad12-SV40).
PWR-1E cells organize into acini and secrete PSA into the lumen, when exposed to androgen. PWR-1E cells organize into acini and secrete PSA into the lumen, when exposed to androgen. When injected with Matrigel or with stromal cells, into athymic male rodents, PWR-1E cells also organize into acini and produce PSA [Webber MM, personal communication].
|
| Complete Growth Medium | The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF).
To make the complete growth medium, you will need to add the following components to the base medium:
|
| Subculturing | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 days Note: Subculture cells before they reach confluence. Do not allow cells to become confluent. |
| Cryopreservation | Freeze Medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 12 D16S539: 12,13 D5S818: 11,13 D7S820: 9,11 THO1: 8,9.3 TPOX: 8,11 vWA: 14,16 |
| Isoenzymes | AK-1, 1 Ref Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420ES-D, 1 Ref Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420G6PD, B Ref Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420GLO-I, 2 Ref Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420Me-2, 0 Ref Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420PGM1, 1 Ref Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420PGM3, 1 Ref Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420 |
| Name of Depositor | Michigan State University, National Cancer Institute |
| Deposited As | human |
| U.S. Patent Number | |
| References | Webber MM, Rhim JS. Human prostatic cell lines immortalized by adenovirus 12-simian virus 40 (AD12/SV40) hybrid virus. US Patent 5,610,043 dated Mar 11 1997 Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420 Okamoto M, et al. Interleukin-6 and epidermal growth factor promote anchorage-independent growth of immortalized human prostatic epithelial cells treated with N-methyl-N-nitrosourea. Prostate 35: 255-262, 1998. PubMed: 9609548 Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636 Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337 Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152 Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497 upon exposure to androgen upregulated upon exposure to androgen Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420 |
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