| 產(chǎn)品名稱 | QNR/K2 |
|---|---|
| 商品貨號 | B165546 |
| Organism | Coturnix coturnix japonica, quail, Japanese |
| Tissue | neuroretina |
| Cell Type | infected with Rous sarcoma virus mutant ts NY-68 |
| Product Format | frozen |
| Morphology | mixed |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | sarcoma |
| Age | embryo, 7 days gestation |
| Applications | Neuroretina (NR) is an evagination of the central nervous system (CNS) which is composed of photoreceptors; glial (Muller) cells; horizontal, bipolar, amacrine; and ganglion neuronal cells. The cells were able to respond to electrical stimulation by producing action potentials which were inhibited by 10(-7) M tetrodotoxin. The specific activity of glutamic acid decarboxylase (GAD), the enzyme responsible for the synthesis of the neurotransmitter gamma-aminobutyric acid, was high. |
| Storage Conditions | liquid nitrogen vapor phase |
| Comments | Neuroretinas were dissected from normal quail embryos, dissociated and immortalized by infection with Rous sarcoma virus (RSV) mutant ts NY-68 to establish NR cell lines. Cells were subsequently cloned after 18 months in culture to establish the cloned cell line QNR/K2. Neuroretina (NR) is an evagination of the central nervous system (CNS) which is composed of photoreceptors; glial (Muller) cells; horizontal, bipolar, amacrine; and ganglion neuronal cells. The QNR/K2 cell line displays properties of Muller (astroglia) cells. The cells were able to respond to electrical stimulation by producing action potentials which were inhibited by 10(-7) M tetrodotoxin. The specific activity of glutamic acid decarboxylase (GAD), the enzyme responsible for the synthesis of the neurotransmitter gamma-aminobutyric acid, was high. QNR/D cells (ATCC CRL-2532) and QNR/K2 cell (ATCC CRL-2533) were transplanted into chicken embryo eyes. Implanted QNR/D cells migrate only to the retinal ganglion and amacrine cell layers and project neurites in the plane of retina. In contrast, QNR/K2 cells migrate through the ganglion and amacrine layers, locate in the inner nuclear layer, and project processes across the retina. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation | Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 39°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor | B Pessac, D Trisler |
| Deposited As | Coturnix coturnix japonica |
| References | Pessac B, et al. A neuronal clone derived from a Rous sarcoma virus-transformed quail embryo neuroretina established culture. Nature 302: 616-618, 1983. PubMed: 6300691 Pessac B, et al. Cells with neuronal properties in permanent cultures of quail embryo neuroretinas infected with Rous sarcoma virus. Brain Res. 275: 53-59, 1983. PubMed: 6313126 Trisler D, et al. Retinal engineering: engrafted neural cell lines locate in appropriate layers. Proc. Natl. Acad. Sci. USA 93: 6269-6274, 1996. PubMed: 8692804 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
