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QT6
QT6
規(guī)格:
貨期:
編號:B165547
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 QT6
商品貨號 B165547
Organism Coturnix coturnix japonica, quail, Japanese
Cell Type methylcholanthrene induced
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease fibrosarcoma
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Derivation Derived from chemically induced tumors of Japanese quail.
Tumorigenic Yes, in animal host.
Effects Yes, in animal host and soft agar.
Virus Susceptibility Rous sarcoma virus
Avian encephalomyelitis virus
Rabies virus
Marek's disease virus
Complete Growth Medium Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 10% tryptose phosphate broth and 5% bovine calf serum
Subculturing Resuspend cells by pipetting fresh medium across the monolayer. Cells may also be dislodged by hitting culture flask against the palm of the hand. Subculture at approximately 75% confluency. Use of 0.5% polyvinylpyrrolidone (PVP) in a trypsin-EDTA solution increases the viability when subculturing by enzymatic means. Neutralize and centrifuge to remove additive.
Note: When subcultures are prepared using pipetting or mechanical means the cells adhere lightly to the flask substrate and exhibit a tendency toward clumping. 
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation Complete growth medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor C Moscovici
Deposited As Coturnix coturnix japonica
References

Moscovici C, et al. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell 11: 95-103, 1977. PubMed: 194709

Imagawa S, et al. Negative regulation of the erythropoietin gene expression by the GATA transcription factors. Blood 89: 1430-1439, 1997. PubMed: 9028967

Martin PT, Sanes JR. Integrins mediate adhesion to agrin and modulate agrin signaling. Development 124: 3909-3917, 1997. PubMed: 9367446

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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