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R1-2
R1-2
規(guī)格:
貨期:
編號(hào):B165551
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 R1-2
商品貨號(hào) B165551
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with the Peyer's patch HEV binding lymphoma line TK1.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against LPAM-1 (lymphocyte Peyer's patch HEV adhesion molecule), VLA-4
Cellular Products
immunoglobulin; monoclonal antibody; against LPAM-1 (lymphocyte Peyer's patch HEV adhesion molecule), VLA-4
Comments

The antibody recognizes the alpha subunit of a 160000/130000 dalton cell surface heterodimer expressed on lymphocytes and lymphomas capable of binding to high endothelial venules (HEV) of murine Peyer's patch.

This subunit corresponds to the alpha subunit of the human VLA-4 integrin receptor, and the two are structurally closely related (if not identical).

Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at cell concentrations between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Isotype IgG2b
Name of Depositor IL Weissman
Deposited As rat (B cell); mouse (myeloma)
References

Holzmann B, et al. Identification of a murine Peyer's patch-specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4alpha. Cell 56: 37-46, 1989. PubMed: 2463092

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
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