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RPC5.4
RPC5.4
規(guī)格:
貨期:
編號(hào):B165623
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 RPC5.4
商品貨號(hào) B165623
Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease plasmacytoma; myeloma
Strain BALB/c
Applications
The line was established from the RPC-5 mineral oil induced BALB/c plasmacytoma.
The cells are killed by 10 nM dexamethasone and are resistant to 0.1 mM 8-azaguanine.
Tested and found negative for ectromelia virus (mousepox).
Karyotype modal number = 60
Derivation
The line was established from the RPC-5 mineral oil induced BALB/c plasmacytoma.
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody,H-2d
Cellular Products
immunoglobulin; monoclonal antibody
Comments
The line was established from the RPC-5 mineral oil induced BALB/c plasmacytoma.
The cells are killed by 10 nM dexamethasone and are resistant to 0.1 mM 8-azaguanine.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a; kappa light chain
Name of Depositor B Mohit
Deposited As Mus musculus
References

Mohit B, Fan K. Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma. Science 171: 75-77, 1971. PubMed: 4099030

Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Schwaber J. Human lymphocyte -- mouse myeloma somatic cell hybrids: selective hybrid formation. Somatic Cell Genet. 3: 295-302, 1977. PubMed: 305122

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