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RPMI 7666 [Gerner 7666]
RPMI 7666 [Gerner 7666]
規(guī)格:
貨期:
編號:B165629
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 RPMI 7666 [Gerner 7666]
商品貨號 B165629
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age 32 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Antigen Expression
HLA A3, Aw29, B7, B12; Blood type B+
Genes Expressed
immunoglobulin; Epstein-Barr virus (EBV)
Cellular Products
immunoglobulin; Epstein-Barr virus (EBV)
Comments
Biological Safety CLASS II.
EBNA positive.
The cells produce Epstein-Barr virus.
Complete Growth Medium RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Culture Conditions Temperature: 37°C
Atmosphere: Air, 95%; CO2, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 9,11
D5S818: 11,12
D7S820: 8,11
THO1: 7,9.3
TPOX: 8,11
vWA: 14,16
Name of Depositor GE Moore
Deposited As Homo sapiens
References

Moore GE, et al. Culture of normal human leukocytes. JAMA 199: 519-524, 1967. PubMed: 4960081

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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