| 產(chǎn)品名稱(chēng) |
S1A(Thy-1-b) |
| 商品貨號(hào) |
B165644 |
| Organism |
Mus musculus, mouse |
| Cell Type |
T lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Strain |
BALB/c |
| Applications |
This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27). The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins. The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine. |
| Derivation |
This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27). |
| Genes Expressed |
The cells secrete a mutant Thy-1 glycoprotein. |
| Cellular Products |
The cells secrete a mutant Thy-1 glycoprotein. |
| Comments |
This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27). The mutation affects the biosynthesis and/or addition of the glycophospholipid anchor. The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins. The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor |
R Hyman |
| Deposited As |
Mus musculus |
| References |
Conzelmann A, et al. No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes. Mol. Cell. Biol. 8: 674-678, 1988. PubMed: 2895421
|
