| 產(chǎn)品名稱(chēng) |
S9.6 |
| 商品貨號(hào) |
B165658 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
BALB/c |
| Applications |
This hybridoma produces a monoclonal antibody, mAb S9.6, which detects DNA/RNA hybrids. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Originally isolated by Carrico and colleagues in 1985. This hybridoma has been recloned and verified as making the intended monoclonal antibody. |
| Genes Expressed |
mAb S9.6 |
| Cellular Products |
This hybridoma expresses a monoclonal antibody, mAb S9.6, which detects DNA/RNA hybrids. |
| Comments |
Mouse (BALB/c) B-cell fused with Mouse (BALB/c) Sp2/0–Ag14 myeloma to produce monoclonal antibodies against RNA-DNA hybrids. Animals were immunized with RNA-DNA hybrids formed by using single stranded PhiX174 DNA as template for E. coli DNA dependent RNA polymerase. The mAb product of this cell line may be used for microarray detection without amplification of RNA; detection of R-loops.
Immunogen: DNA-RNA hybrids synthesized from ?Χ174 single stranded-DNA.
Primary reactivity: DNA-RNA hybrids |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL. Maintain cultures at a cell concentration between 5 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Fetal bovine serum, 90%; DMSO, 10%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Isotype |
IgG2a Kappa light chain |
| Name of Depositor |
SH Leppla |
| References |
Hu, et al. An antibody-based microarray assay for small RNA detection. Nucleic Acids Res 34(7):352, 2006.
Boguslawski S, et al. Characterization of monoclonal antibody to DNA.RNA and its application to immunodetection of hybrids.J Immunol Methods 89(1):123-30, 1986.
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