| 產(chǎn)品名稱 |
SC |
| 商品貨號 |
B165667 |
| Organism |
Homo sapiens, human |
| Tissue |
peripheral blood |
| Cell Type |
monocyte/macrophage |
| Product Format |
frozen |
| Morphology |
monocyte/macrophage |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
The SC cell line was established from human peripheral blood mononuclear cells. |
| Comments |
The cells grow as single cells in suspension, and have a granular appearance. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium.
Interval: Maintain the cell concentration between 2 X 105 and 1 X 106 cells/mL
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
This cell line was originally deposited in support of a patent (US Patent 5,447,861).
STR profiling at ATCC indicates similarity to other cell lines including:
U-937 (ATCC CRL-1593.2) and
EL 1 (ATCC CRL-9854) |
| Name of Depositor |
E.I. du Pont de Nemours & Co. |
| Deposited As |
Homo sapiens |
| U.S. Patent Number |
|
| References |
Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995
|
