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SK-UT-1B
SK-UT-1B
規(guī)格:
貨期:
編號:B165724
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 SK-UT-1B
商品貨號 B165724
Organism Homo sapiens, human
Tissue uterus; endometrium
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease grade III,mesodermal tumor (mixed); consistent with leiomyosarcoma
Age 75 years
Gender female
Ethnicity Caucasian
Karyotype (P27) hypodiploid to diploid
Clinical Data
75 years
Caucasian
female
Antigen Expression
Blood type B; Rh+
Genes Expressed
Blood type B; Rh+
Tumorigenic Yes
Effects
Yes, in nude mice
Comments

This is a substrain of SK-UT-1 (see ATCC HTB-114) which reportedly differs in morphology, karyology and in the type of tumor which develops on animal inoculation.

The cells form well differentiated adenocarcinomas consistent with endometrial carcinoma.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 10,13
D16S539: 12,14
D5S818: 10,11
D7S820: 9,10
THO1: 7
TPOX: 8
vWA: 16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1-2
PGM1, 1
PGM3, 1
Name of Depositor G Trempe
Deposited As Homo sapiens
References

Chen TR. SK-UT-1B, a human tumorigenic diploid cell line. Cancer Genet. Cytogenet. 33: 77-81, 1988. PubMed: 3383166

Chen TR. SK-UT-1B cell line has the diploid karyotype. A reply. Cancer Genet. Cytogenet. 48: 139-141, 1990. PubMed: 2372783

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

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