| 產品名稱 | Sl/Sl4 hSCF220 |
|---|---|
| 商品貨號 | B165727 |
| Organism | Mus musculus, mouse |
| Tissue | liver |
| Cell Type | Mast Cell |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 2 Papovavirus (Cells may contain SV40 viral sequences)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | embryo |
| Applications | SL/Sl4 hSCF220 is a transfected SL/Sl4 cell line that expresses membrane-bound stem cell factor (SCF).
This cell line was derived from the Sl/Sl4 cell line (ATCC CRL-2452) by transfection with spliced forms of hSCF cDNAs inserted into the pJT-1 (hSCF220) vector and expressed off the simian virus 40 (SV40) early promoter.
Defects in the HM, associated with the Steel (Sl) mutation in
mice, have been shown to be due to abnormalities in the production or presentation of the protein product of the Steel gene.
Sl/Sl4 is a SV40 large T antigen immortalized stromal cell line derived from the hematopoietic microenvironment (HM) of a fetal murine homozygous (Sl/Sl) SCF-deficient embryo.
It exists as a locally secreted or membrane-bound protein.
SL/Sl4 hSCF220 cells do not secrete detectable amounts of SCF.
Secreted SCF is available in the transfected Sl/Sl4 cell line, Sl/Sl4 hSCF248 (ATCC CRL-2454). |
| Storage Conditions | liquid nitrogen vapor phase |
| Derivation | This cell line was derived from the Sl/Sl4 cell line (ATCC CRL-2452) by transfection with spliced forms of hSCF cDNAs inserted into the pJT-1 (hSCF220) vector and expressed off the simian virus 40 (SV40) early promoter. Sl/Sl4 is a SV40 large T antigen immortalized stromal cell line derived from the hematopoietic microenvironment (HM) of a fetal murine homozygous (Sl/Sl) SCF-deficient embryo. |
| Comments | SL/Sl4 hSCF220 is a transfected SL/Sl4 cell line that expresses membrane-bound stem cell factor (SCF). This cell line was derived from the Sl/Sl4 cell line (ATCC CRL-2452) by transfection with spliced forms of hSCF cDNAs inserted into the pJT-1 (hSCF220) vector and expressed off the simian virus 40 (SV40) early promoter. It was cotransfected with the p48 vector encoding hygromycin B using the Lipofectin method. Defects in the HM, associated with the Steel (Sl) mutation in
mice, have been shown to be due to abnormalities in the production or presentation of the protein product of the Steel gene. Sl/Sl4 is a SV40 large T antigen immortalized stromal cell line derived from the hematopoietic microenvironment (HM) of a fetal murine homozygous (Sl/Sl) SCF-deficient embryo. This product is termed stem cell factor (SCF) or mast cell growth factor (MGF). It exists as a locally secreted or membrane-bound protein. Both forms show biological activity when assayed on human hematopoietic cells. SL/Sl4 hSCF220 cells that express the membrane-bound form (hSCF220) of SCF supported the maintenance of human progenitor cells in culture for up to four weeks This was 1-2 weeks longer than SL/Sl4 hSCF248. SL/Sl4 hSCF220 cells do not secrete detectable amounts of SCF. Secreted SCF is available in the transfected Sl/Sl4 cell line, Sl/Sl4 hSCF248 (ATCC CRL-2354). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Subculture when flasks reach 80% to 90% confluency.
Subcultivation Ratio: 1:10 to 1:30 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation | Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor | DA Williams |
| Deposited As | mouse |
| References | Toksoz D, et al. Support of human hematopoiesis in long-term bone marrow cultures by murine stromal cells selectively expressing the membrane-bound and secreted forms of the human homolog of the steel gene product, stem cell factor. Proc. Natl. Acad. Sci. USA 89: 7350-7354, 1992. PubMed: 1380155 Majumdar MK, et al. Identification and mutation of primary and secondary proteolytic cleavage sites in murine stem cell factor cDNA yields biologically active, cell-associated protein. J. Biol. Chem. 269: 1237-1242, 1994. PubMed: 7507105 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
