| 產(chǎn)品名稱 |
SW 872 [SW-872, SW872] |
| 商品貨號 |
B165786 |
| Organism |
Homo sapiens, human |
| Tissue |
connective tissue |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
liposarcoma |
| Age |
36 years adult |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
hypertriploid; modal number = 80; range = 66 to 81. The rate of higher ploidies was 8.2%.Ten markers were common to most cells. These were: der(5)t(5;?)(q31;?)1, der(5)t(5;?)(q31;?)2, der(6)t(6;?)(q15;?), der(7)t(7;?)(q36;?), t(15q16q) and five others. Both der(5) markers, the der(7) and t(15q16q) were paired.There were 5 copies of N20 and N21, 4 copies of N8, N9, N11, N14 and N17 and a single copy of X in each cell. |
| Derivation |
The SW 872 cell line was initiated by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a fibrosarcoma removed from a 36 year old male Caucasian. |
| Clinical Data |
Caucasian
male
36 years adult
The histopathology evaluation reported an undifferentiated malignant tumor consistent with liposarcoma. |
| Antigen Expression |
Antigen expression: Blood type O+ |
| Tumorigenic |
Yes, |
| Effects |
Yes, produces spindle cell sarcoma in nude mice consistent with liposarcoma |
| Comments |
The histopathology evaluation reported an undifferentiated malignant tumor consistent with liposarcoma. An ampule at passage 4 was received at the ATCC in January, 1982. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
|
| Subculturing |
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 100%
Temperature: 37°C
|
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor |
A Leibovitz |
| Deposited As |
Homo sapiens |
| Passage History |
An ampule at passage 4 was received at the ATCC in January, 1982. |
| Year of Origin |
1974 |
| References |
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Hu M, et al. Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res. 57: 3577-3584, 1997. PubMed: 9270031
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