產(chǎn)品名稱 |
TA 99 |
商品貨號 |
B165823 |
Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
Cell Type |
hybridoma: B lymphocyte |
Product Format |
frozen |
Morphology |
lymphoblast |
Culture Properties |
suspension |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Applications |
This cell line produces a monoclonal antibody which can be used as a control for AS 33 (see ATCC HB-8779). |
Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
Genes Expressed |
immunoglobulin; monoclonal antibody |
Cellular Products |
immunoglobulin; monoclonal antibody |
Comments |
This cell line produces a monoclonal antibody which can be used as a control for AS 33 (see ATCC HB-8779). |
Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine, 1% nonessential amino acids, 100 units/ml penicillin and 0.1 mg/ml streptomycin, 90%; fetal bovine serum, 10%
|
Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
Cryopreservation |
culture medium, 90%; DMSO, 10% |
Isotype |
IgG2a; kappa light chain |
Name of Depositor |
Sloan-Kettering Institute for Cancer Research |
Deposited As |
mouse (B cell); mouse (myeloma) |
U.S. Patent Number |
|
References |
Welt S, et al. Method of imaging colorectal carcinoma lesion and composition for use therein. US Patent 5,160,723 dated Nov 3 1992
|