| 產(chǎn)品名稱(chēng) |
TCCSUP |
| 商品貨號(hào) |
B165830 |
| Organism |
Homo sapiens, human |
| Tissue |
urinary bladder |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
grade IV transitional cell carcinoma |
| Age |
67 years |
| Gender |
female |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
(P12 and 35) hypotetraploid with marker chromosomes |
| Derivation |
The TCCSUP line was isolated in 1974 from an anaplastic transitional cell carcinoma (TCC) in the neck of the urinary bladder. |
| Clinical Data |
female
|
| Antigen Expression |
HLA 2, 3, 7, 12 |
| Comments |
The patient had a 4 month history of hematuria prior to removal of the tumor.
Metastases to the bone marrow were discovered later.
Studies on ultrastructure indicated presence of microvilli and lipid bodies but no desmosomes were observed. |
| Complete Growth Medium |
Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids (ATCC 30-2003) and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
|
| Subculturing |
- Remove and discard culture medium.
- Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting
- Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 11,14 D16S539: 9,11 D5S818: 12 D7S820: 8,9 THO1: 6,9.3 TPOX: 8 vWA: 14,16 |
| Isoenzymes |
AK-1, 1-2 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 2 PGM3, 1 |
| Name of Depositor |
C O'Toole |
| References |
O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.
Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484
Nayak SK, et al. A cell line from an anaplastic transitional cell carcinoma of human urinary bladder. Br. J. Cancer 35: 142-151, 1977. PubMed: 836756
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