| 產(chǎn)品名稱 |
TGP47 |
| 商品貨號 |
B165854 |
| Organism |
Mus musculus, transgenic, mouse, transgenic |
| Tissue |
pancreas |
| Cell Type |
Epithelial |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 CELLS CONTAIN PAPOVAVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
pancreatic acinar cell tumor; carcinoma |
| Age |
26 weeks |
| Gender |
male |
| Strain |
Tg(E1a-1-SV40E)Bri18 |
| Applications |
TGP47 is an epithelial like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse. The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen. The cells do not secrete amylase or lipase. Histochemical staining of the current stock of TGP47 was negative for somatostatin and positive for trypsin. |
| Derivation |
TGP47 is an epithelial like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse. |
| Clinical Data |
TGP47 is an epithelial like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse. male |
| Comments |
TGP47 is an epithelial like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse. The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen. TGP47 cells appear somewhat duct like in culture, but contain rare somatostatin positive cells. The cells do not secrete amylase or lipase. Histochemical staining of the current stock of TGP47 was negative for somatostatin and positive for trypsin. Morphologic and differentiation markers of this line appear to fluctuate from time to time in culture. |
| Complete Growth Medium |
A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. -
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Twice per week |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Name of Depositor |
OS Pettengill, D Longnecker |
| Deposited As |
mouse, transgenic |
| References |
Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904
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