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TIMI.4
TIMI.4
規(guī)格:
貨期:
編號:B165864
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 TIMI.4
商品貨號 B165864
Organism Mus musculus, mouse
Tissue thymus
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease lymphoma
Strain C57BL/6
Applications
Derived from a radiation leukemia virus induced thymoma.
The line is sensitive to cortisol (0.001 mM) and PHA (0.02 mg/ml).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Derived from a radiation leukemia virus induced thymoma.
Antigen Expression
H-2b; Thy-1.2
Genes Expressed
H-2b; Thy-1.2
Comments
Derived from a radiation leukemia virus induced thymoma.
The line is sensitive to cortisol (0.001 mM) and PHA (0.02 mg/ml).
TL antigen is not expressed.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor R Hyman
Deposited As Mus musculus
References

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Hyman R. Studies on surface antigen variants. Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

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