| 產(chǎn)品名稱 |
U-138 MG |
| 商品貨號 |
B165896 |
| Organism |
Homo sapiens, human |
| Tissue |
brain |
| Cell Type |
glioblastoma |
| Product Format |
frozen |
| Morphology |
polygonal |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
glioblastoma; classified as grade IV as of 2007 |
| Age |
47 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Karyotype |
Hyperdiploid to pentaploid with several markers; the stemline chromosome number is near triploid with the 2S component occurring at 9.8%., Five markers [t(11;5), t(8q;4), t(19;?18), M1 and M2] were common to most S metaphases. One chromosome 4 could be found in every S metaphase. Chromosome composition was very uniform among cells. |
| Derivation |
This is one of a number of cell lines derived from malignant gliomas (see also ATCC HTB-14 and ATCC HTB-15 ) by J. Ponten and associates from 1966 to 1969.- |
| Clinical Data |
47 years Caucasian male |
| Antigen Expression |
Blood Type A; Rh+ |
| Tumorigenic |
No |
| Effects |
No, in immunosuppressed mice Yes, in semisolid medium |
| Comments |
It differs from ATCC HTB-14 in morphology and it has a slower proliferation rate.
Mycoplasma contamination was observed and cured by March 1974. NOTE: The two glioblastoma cell lines, U-118 MG (HTB-15) and U-138 MG (HTB-16), reportedly from different individuals have identical VNTR and similar STR patterns.
U-118 MG and U-138 MG are very similar cytogenetically and share at least six derivative marker chromosomes. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
|
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile |
Amelogenin: X,Y CSF1PO: 12 D13S317: 9,11 D16S539: 12,13 D5S818: 11 D7S820: 9 THO1: 6 TPOX: 8 vWA: 18 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor |
J Ponten |
| Deposited As |
Homo sapiens |
| Year of Origin |
1966 |
| References |
Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504
Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765
Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760
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