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UC7-13D5
UC7-13D5
規(guī)格:
貨期:
編號:B165906
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

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凍干粉
斜面
甘油
平板


產品名稱 UC7-13D5
商品貨號 B165906
Organism Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with the murine T cell receptor gamma/delta TCR.
The antibody is specific for all murine TCR gamma/delta heterodimers.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR)
Comments
Animals were immunized with the G8 mouse T cell clone (TCR gamma/ delta).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the murine T cell receptor gamma/delta TCR.
The antibody is specific for all murine TCR gamma/delta heterodimers.
Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Two to three times weekly.
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG
Name of Depositor JA Bluestone
Deposited As hamster, Armenian (B cell); mouse (myeloma)
References

Bluestone JA, et al. Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunol. Rev. 120: 5-33, 1991. PubMed: 1650760

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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