| 產(chǎn)品名稱 |
WEHI 22.1 |
| 商品貨號 |
B165937 |
| Organism |
Mus musculus, mouse |
| Tissue |
thymus |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Strain |
BALB/c |
| Applications |
The line was derived from a thymoma induced by X-irradiation. They are sensitive to 100 nM cortisol. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
The line was derived from a thymoma induced by X-irradiation. |
| Antigen Expression |
Thy-1.2 |
| Genes Expressed |
immunoglobulin; monoclonal antibody,Thy-1.2 |
| Cellular Products |
immunoglobulin; monoclonal antibody |
| Comments |
The line was derived from a thymoma induced by X-irradiation. The cells have characteristics of both T and B cells and form rosettes with sheep erythrocytes. They are sensitive to 100 nM cortisol. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgM (surface) |
| Name of Depositor |
AW Harris |
| Deposited As |
Mus musculus |
| References |
Harris AW, et al. Differentiated functions expressed by cultured mouse lymphoma cells. II. theta Antigen, surface immunoglobulin and a receptor for antibody in cells of a thymoma cell line. J. Immunol. 110: 431-438, 1973. PubMed: 4630643
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