| 產(chǎn)品名稱 |
WEHI-274.1 |
| 商品貨號 |
B165942 |
| Organism |
Mus musculus, mouse |
| Cell Type |
monocyte |
| Product Format |
frozen |
| Morphology |
monocyte |
| Culture Properties |
suspension |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Abelson murine leukemia virus-induced tumor |
| Strain |
BALB/c |
| Applications |
The line expresses the MAC-1 antigen, does not express Ia and is not phagocytic. Tested and found negative for ectromelia virus (mousepox). |
| Antigen Expression |
MAC-1 antigen (CD11b) |
| Receptor Expression |
Fc |
| Genes Expressed |
MAC-1 antigen (CD11b) |
| Comments |
The line expresses the MAC-1 antigen, does not express Ia and is not phagocytic. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; fetal bovine serum, 10%; 0.05 mM 2-mercaptoethanol
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Adherent cells can be dislodged by scraping. Start new cultures at 1 X 10 exp5 cells/ml and maintain at or below 1 X 10 exp6 cells/ml. |
| Name of Depositor |
NL Warner, LL Lanier |
| Deposited As |
Mus musculus |
| References |
. Immunobiology and immunotherapy of cancer. New York: Elsevier/North Holland; 1979.
Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787
|
