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Wgd5
Wgd5
規(guī)格:
貨期:
編號(hào):B165946
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Wgd5
商品貨號(hào) B165946
Organism Mus musculus, mouse
Tissue connective tissue
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Leukemia
Applications
transfection host
Helper cell line for packaging retroviral vectors such as those released from lambdaZd31, lambdaZd32, and lambdaZd35 (ATCC 37563-5, respectively).
Derivation
The line was derived from the WOP cell line (a 3T3 derivative transformed by an origin defective polyomavirus).
Genes Expressed
retrovirus vector (Moloney murine leukemia virus
Cellular Products
retrovirus vector (Moloney murine leukemia virus
Comments
The Wgd5 cell line contains a mutant of the Moloney murine leukemia virus (MoMuLV) that is useful for packaging recombinant retroviral DNA into infectious virus particles.
The line was derived from the WOP cell line (a 3T3 derivative transformed by an origin defective polyomavirus).
Wgd5 was selected as a gpt+ clone after cotransformation of WOP with pSV2gpt and a packaging mutant of MoMuLV.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 0.25 mg/ml xanthine, 0.015 mg/ml hypoxanthine, 0.01 mg/ml thymidine, 0.002 mg/ml aminopterin and 0.015 mg/ml mycophenolic acid, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, rinse and remove trypsin.
Allow the cells to sit at 37C until they begin to detach (about 3 to 5 minutes), add fresh medium, aspirate and dispense into new flasks.
Subculture before the cells become confluent.
If allowed to become confluent, the cells will slough off and die.
Name of Depositor AJ Murphy
Deposited As Mus musculus
References

Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791

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