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XVI E6E6G10
XVI E6E6G10
規(guī)格:
貨期:
編號:B165977
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 XVI E6E6G10
商品貨號 B165977
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody can be used to assay 1,25-dihydroxy vitamin D.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against porcine (pig) receptor for 1,25-dihydroxy vitamin D3
Cellular Products
immunoglobulin; monoclonal antibody; against porcine (pig) receptor for 1,25-dihydroxy vitamin D3
Comments
The antibody can be used to assay 1,25-dihydroxy vitamin D.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 1 mM sodium pyruvate,
2 mM L-glutamine, 10 mM nonessential amino acids, 10 mM HEPES and 50 uM
2-mercaptoethanol, 85%; fetal bovine serum, 15%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/ml and maintain between 1 X 105 and 1 X 106 cells/ml.
Cryopreservation
fetal bovine serum, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor Wisconsin Alumni Res. Fndn.
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Dame MC, et al. Monoclonal antibodies to the porcine intestinal receptor for 1,25-dihydroxyvitamin D3: Interaction with distinct receptor domains. Biochemistry 25: 4523-4534, 1986. PubMed: 2429689

Dame MC, et al. Assay for 1,25-dihydroxy vitamin D in sample containing vitamin D transport protein. US Patent 4,816,417 dated Mar 28 1989

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