| 產(chǎn)品名稱 | Aspergillus terreus Thom |
|---|---|
| 商品貨號 | B166813 |
| Strain Designations | MF4845 |
| Application | Produces hypocholesterolemic agents
Produces hypolipemic agents
Produces lovastatin
Produces mevinolin
Produces mevinolinic acid |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | freeze-dried |
| Storage Conditions | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain | no |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Preceptrol® | no |
| Genome Sequenced Strain | Yes |
| Comments | Genome sequencing strain (Microbia, Inc., USA) |
| Medium | ATCC® Medium 200: YM agar or YM broth ATCC® Medium 324: Malt extract agar ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions | Temperature: 24°C to 26°C
Atmosphere: Typical aerobic |
| Sequenced Data |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGAAGGATCATTACCGAGTGCGTGTCTTTATGGCCCAACCTCCCACCCGTGACTATTGTACCTTGTTGCTTCGGCGGGCCCGCCAGCGTTGCTGGCCGCCGGGGGGCGACTCGCCCCCGGGCCCGTGCCCGCCGGAGACCCCAACATGAACCCTGTTCTGAAAGCTTGCAGTCTGAGTGTGATTCTTTGCAATCAGTTAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCCCGGCTTGTGTGTTGGGCCCTCGTCCCCCGGCTCCCGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGGTCCTCGAGCGTATGGGGCTTCGTCTTCCGCTCCGTAGGCCCGGCCGGCGCCCGCCGACGCATTTATTTGCAACTTGTTTTTTTCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCA D1D2 region of the 28S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGCTGGCTCCTTCGGGGTCCGCATTGTAATTTGCAGAGGATGCTTCGGGTGCAGCCCCCGTCTAAGTGCCCTGGAACGGGCCGTCATAGAGGGTGAGAATCCCGTATGGGGCGGGGTGTCTGCGTCCGTGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCAACCAGACTCGCTCGCGGGGTTCAGCCGGGCTTCGGCCCGGTGTACTTCCCCGCGGGCGGGCCAGCGTCGGTTTGGGCGGCCGGTCAAAGGCCTCCGGAATGTAGCGCCCTTCGGGGCGCCTTATAGCCGGGGGTGCAATGCGGCCAGCCTGGACCGAGGAACGCGCTTCGGCACGGACGCTGGCATAATGGTTGTAAACGA beta-tubulin gene TGTGCCCGATCCCCCTGTTCCCCTCTCCCCCGACGCGTCTCTTTTGGGCTGCTCTCTGAAGTCCAACCCCACCCGTTCCTGGAGAAACTTTGGATGAATTGACTAACATTCTTTCTTCTTCGTGATCATAGGTTCATCTGCAAACCGGCCAGTGTGTAAGTGCGACCGTGTCCTTTCGATGAGGATGGGAATAGGATGTTTTGTGACAGAGACTGAACTGGGTGGTGGTGATAGGGTAACCAAATTGGTGCCGCTTTCTGGTACGTCTGGAATCAACCTGGGGAATGCTGGCTCTCGTGGGATGCAGAGTCTTACGGACATGCGTCCTCGGGCTTAAAAGGGTTCCGTGGTGGCATGATGCTGACAACTGTACAGGCAAACCATCTCTGGCGAGCACGGCCTTGATGGCTCCGGTGTGTAAGTGTCACCGACGCCCGCTCAATGGGCTCCCATAATAGAGGATTACATGACGATGGACGATTCTGATGTGGAAACAGCTTCAATGGCTCCTCCGACCTCCAGCTCGAGCGCATGAACGTCTACTTCAACGAGGTACGTCCCTTCCACACCATCCTGTGACAGATTCTCCACGCTTAAAAGACCTCGACACTAATTTCCGATCCCCTTTAGGCCAGCGGAAACAAGTATGTTCCTCGTGCTGTCCTCGTTGACCTTGAGCCCGGTACCATGGACGCCGTCCGTGCCGGTCCCTTCGGTCAGCTCTTCCGTCCCGACAACTTCGTCTTTGGCCAGTCTGGTGCCGGTAACAACTGG |
| Name of Depositor | Merck & Co., Inc. |
| U.S. Patent Number | |
| Cross References | Nucleotide (GenBank) : GU256759 ITS including 5.8S rRNA gene Nucleotide (GenBank) : AABT00000000 Aspergillus terreus ATCC 20542, whole genome shotgun sequencing project |
| References | Greenspan MD, Yudkovitz JB. Mevinolinic acid biosynthesis by Aspergillus terreus and its relationship to fatty acid biosynthesis. J. Bacteriol. 162: 704-707, 1985. PubMed: 3988710 Moore RN, et al. Biosynthesis of the hypocholesterolemic agent mevinolin by Aspergillus terreus. Determination of the origin of carbon, hydrogen, and oxygen atoms by 13C NMR and mass spectrometry. J. Am. Chem. Soc. 107: 3694-3701, 1985. Lam TY. Hypocholesterolemic fermentation products. US Patent 4,376,863 dated Mar 15 1983 Hajko P, et al. Process for the isolation of lovastatin. US Patent 5,712,130 dated Jan 27 1998 Alberts AW, et al. Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent. Proc. Natl. Acad. Sci. USA 77: 3957-3961, 1980. PubMed: 6933445 Askenazi M, et al. Integrating transcriptional and metabolite profiles to direct the engineering of lovastatin-producing fungal strains. Nat. Biotechnol. 21: 150-156, 2003. Schierova M, Linka M, Pazoutova S. Sulfate assimilation in Aspergillus terreus: analysis of genes encoding ATP-sulfurylase and PAPS-reductase. Curr. Genet. 38: 126-131, 2000. PubMed: 11057445 Hendrickson L, et al. Lovastatin biosynthesis in Aspergillus terreus: characterization of blocked mutants, enzyme activities and a multifunctional polyketide synthase gene. Chem. Biol. 6: 429-439, 1999. PubMed: 10381407 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
