Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 6.763, 3.407, 5.813, 2.064, 3.028, 2.505, 3.971, 0.941, 1.169, 1.031, 1.203, 0.520, 2.072, 1.938. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.48, 0.92, 1.0, 1.2, 1.9, 2.1, 2.6, 3.2, 3.6, 4.0, 6.1, 7.4. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70193, 70882, 70894, 70895, 71124. |