Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--2.9(doublet), 1.1, 0.62; EcoRI--3.0, 2.0, 1.6, 0.94; NotI--7.4. Insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.27; EcoRI--0.94, 2.54; HindIII--0.92, 1.62, 2.75, 3.16; KpnI--1.56; NcoI--1.59; NotI--0.27; PstI--1.85, 2.35, 2.86, 3.28; PvuII--3.24; SphI--1.09; XbaI-1.70. |