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pHE-A1 [he-A1]
pHE-A1 [he-A1]
規(guī)格:
貨期:
編號:B220930
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pHE-A1 [he-A1]
商品貨號 B220930
Designations pHE-A1 [he-A1]
Species Homo sapiens, human
Depositors NK Spurr
Vector
Construct size (kb): 8.100000381469727
Insert
DNA: genomic
Insert lengths(kb): 3.900000095367432
Gene product: thyroid hormone receptor, alpha 1 (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 1, formerly ERBA1) [ERBA1]
Alleles: A1, A1, A2, A2
Insert Size (kb) 3.900
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 4.2; HindIII--4.8, 2.8; BamHI--8.3.
A 2.9 kb EcoRI/HindIII subfragment detects a PvuII RFLP.
Insert includes 2.4 kb of unique sequence and a 1.5 kb HindIII/EcoRI fragment containing repetitive sequence.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
The PvuII polymorphism has demonstrated co-dominant segregation in 2 families. Only one allele is present in mouse/human hybrids with one chromosome 17.
This is a subclone of lambdahe-A1 (p01239).
Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, HinfI, KpnI, PstI, TaqI.
References

Jansson M, et al. Isolation and characterization of multiple human genes homologous to the oncogenes of avian erythroblastosis virus. EMBO J. 2: 561-565, 1983. PubMed: 6313346

Spurr NK, et al. Chromosomal localisation of the human homologues to the oncogenes erbA and B. EMBO J. 3: 159-163, 1984. PubMed: 6323162

Rider SH, et al. RFLP for the human erb-A1 gene. Nucleic Acids Res. 15: 863, 1987. PubMed: 2881264

Douglas JB, et al. A pulsed-field gel electrophoresis map locates the polymorphic probes for ERBA2 and ErbAbeta within 120 kb of each other, confirming that THRB (formerly ERBA2) maps to chromosome 3. Genomics 9: 398-400, 1991. PubMed: 1672299

Barbara Weiffenbach, personal communication

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