| 產(chǎn)品名稱 |
pRRS-SacIR+ [NEB 963] |
| 商品貨號(hào) |
B221287 |
| Designations |
pRRS-SacIR+ [NEB 963] |
| Species |
Streptomyces achromogenes Okami and Umezawa |
| Depositors |
New England Biolabs, Inc., GD Williams, New England Biolabs, Inc. |
| Applications |
This is an E. coli containing 3 plasmids - pRRS-SacIR+, pLG-SacIM+ and pLG339- that produces the restriction endonuclease and modification methylase SacI. |
| Insert |
DNA: genomic Gene product: restriction endonuclease SacI Gene product: modification methylase SacI |
| Media |
ATCC Medium 1065 (see below) plus ampicillin (100.0 ug/ml) plus kanamycin (50.0 ug/ml) plus tetracycline (10.0 ug/ml)
ATCC Medium 1065:
Tryptone (Difco 0123), 10.0 g
Yeast Extract (Difco 0127), 5.0 g
NaCl, 10.0 g
Distilled water, 1.0 L
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Shipped: ?Freeze dried E. coli containing the plasmids |
| Comments |
This is an E. coli containing 3 plasmids - pRRS-SacIR+, pLG-SacIM+ and pLG339- that produces the restriction endonuclease and modification methylase SacI. This should be grown on LB media containing ampicillin (50 ug/ml), kanamycin (50 ug/ml) and tetracycline (15 ug/ml) to maintain all plasmids. This culture must be grown at temperatures no greater than 30C to maintain stability. |
| References |
Xu SY, Xiao J. Method for cloning and producing the SacI restriction endonuclease. US Patent 5,532,153 dated Jul 2 1996
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
