Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; HindIII--4.4, 1.4; EcoRI/HindIII--4.4, 1.4; PstI--6.0; BamHI--6.0. This probe shows linkage to SCA1. The BamHI polymorphism is in linkage disequilibrium with the EcoRV polymorphism. Enzyme(s) not detecting polymorphism: MspI, TaqI, BclI, BglII, PvuII, EcoRV. |