Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--11.0, 4.0, 1.2; PstI--7.2, 4.1, 3.9, 1.0, 0.8; XbaI--11.0, 3.5, 1.6; BamHI/SalI--7.4, 4.0, 2.7, 1.3, 1.0. The insert contains the following restriction sites (approximate kb from the end distal to the vector SphI site): BamHI--0.4, 1.6, 5.3; XbaI--0.8, 3.9; SphI--1.3; EcoRI--1.9, 2.0. |