| 產(chǎn)品名稱 |
Influenza A virus (H3N2) |
| 商品貨號 |
B221857 |
| Classification |
Orthomyxoviridae, Alphainfluenzavirus |
| Agent |
Influenza A virus (H3N2) |
| Strain |
A/Aichi/2/68 |
| Applications |
Emerging infectious disease research Respiratory research Influenza Research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Comments |
Derived by adaptation from allantoic product ATCC® VR-547™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-547™ and ATCC® VR-1680™ have not been compared for sequence or infectivity in chicken embryo culture and tissue culture. |
| Effect on Host |
CPE, rounding and degeneration |
| Recommended Host |
MDCK (ATCC® CCL-34™) |
| Growth Conditions |
Temperature: 35°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days |
| Name of Depositor |
MT Coleman, Centers for Disease Control and Prevention; ATCC® |
| Source |
Isolated from sailor on Israeli ship docking in Aichi, Japan, 1968 |
| References |
Coleman MT, et al. The Hong Kong-68 influenza A2 variant. Lancet 2: 1384-1386, 1968. PubMed: 4177941
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