国产黄片大全在线播放_多毛中国农村熟女吟呻对白_欧洲乱码在线播放_亚洲五月婷婷久久综合色

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 94T778
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
94T778
94T778
規(guī)格:
貨期:
編號:B222268
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 94T778
商品貨號 B222268
Organism Homo sapiens, human
Tissue retroperitoneum; recurring
Product Format frozen
Morphology fibroblast-like
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease liposarcoma; well-differentiated
Age 69 years
Gender female
Ethnicity Caucasian
Applications The MDM2 amplification present in this cell line can be used as a model for testing new drugs. This cell line is one of the rare liposarcoma cell lines available.
Storage Conditions liquid nitrogen vapor phase
Karyotype 48~51,XX,-7,-22,+32~6r,+mar[cp10]/49~52,XX,del(7)(q),-22, +3~6r,+mar[cp3] . ish (WCP1+;WCP12+). ish mar(WCP1+;WCP12+)
Images 94T778 ATCC CRL-3044 Cell Micrograph
Derivation The 94T778 cell line is a well-differentiated liposarcoma derived from the retroperitoneum of a 69 year old female. It was established from the second recurrence of the tumor from the same individual from which the 93T449 cell line (ATCC CRL-3043) was derived.
Oncogene MDM2 (murine double minute 2); CDK4 (cyclin-dependent kinase 4); HMGA2 (high mobility group AT-hook 2)
Comments Karyotypic and molecular characterization of this liposarcoma demonstrates the presence of ring and large marker chromosomes containing MDM2, CDK4 and HMGA2 amplification.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  7. Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended.
Medium renewal: every 2 to 3 days
Cryopreservation Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile CSF1PO: 10, 11
D13S317: 8, 14
D16S539: 12, 13
D5S818: 12
D7S820: 10
TH01: 8, 9
TPOX: 8, 11
vWA: 14, 17
Amelogenin: X
Name of Depositor F Pedeutour
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479