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<em>Saccharomyces cerevisiae</em> CEN BANK genomic library in <em>E. coli </em>
<em>Saccharomyces cerevisiae</em> CEN BANK genomic library in <em>E. coli </em>
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貨期:
編號(hào):B222375
品牌:Mingzhoubio

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產(chǎn)品名稱 Saccharomyces cerevisiae CEN BANK genomic library in E. coli
商品貨號(hào) B222375
Designations Saccharomyces cerevisiae CEN BANK genomic library in E. coli
GenBank Definition

Saccharomyces cerevisiae succinate dehydrogenase (SDHA) gene,

Yeast DNA for CTP: phosphoethanolamine cytidylyltransferase and

Saccharomyces cerevisiae succinate dehydrogenase membrane anchor

Saccharomyces cerevisiae synaptobrevin (SNC2) gene, complete cds.

GenBank Number

M86746

D50644

L26333

L16243

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors MD Rose
Vector
DNA: Genomic
Digest: Partial
Insert size range: 10 - 20 kb
Genome Equivalent: 0.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Frozen aliquots of the library in E. coli
Comments
The vial labeled 95-02A corresponds to Cen Bank A and the vial labeled 95-02B corresponds to Cen Bank B.

Resuspend in a small amount of broth and plate for single colonies to avoid fast growing clones taking over the culture.

For Cen Bank A, the insert range is 10-15 kb, the number of clones for coverage is 8900, and number of independent recombinants is 8.9x103. There are 10% non-recombinants.

For Cen Bank B, the insert range is 15-20 kb, the number of clones for coverage is 9500, and the number of independent recombinants is 9.5x103. There are 10% non-recombinants.

The titer of Cen Bank A is about 1.5 x 109 cfu/mL and the titer of Cen Bank B is about 2.0 x 108 cfu/mL. The library has been amplified once by the ATCC.

GRF88 is an S288C derivative (MATalpha his4-38).
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Thrash C, et al. Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene. Proc. Natl. Acad. Sci. USA 82: 4374-4378, 1985. PubMed: 2989818

Rose MD, et al. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243, 1987. PubMed: 3327750

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