Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 1.5, 0.65; EcoRI--5.4, 1.45; HindIII--3.2, 2.3, 0.85, 0.7; PstI--6.9; PstI/XhoI--3.9, 2.9. The insert contains the following restriction sites (nt from the 5' end): HindIII--85, 856, 1539, 3792; SalI--568; BamHI--1600, 2252; EcoRI--1510. The insert extends from nt 2438-6304 of the reported sequence, including exons 13 through the 3' end. Also includes a short (14 nt) poly(A) tail not in the sequence record. Transcription from the T3 promoter gives the sense strand. |
References |
Oliva D, et al. Complete structure of the human gene encoding neuron-specific enolase. Genomics 10: 157-165, 1991. PubMed: 2045099
Tohru Yorifuji, personal communication
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