| 產品名稱 | VMM425 |
|---|---|
| 商品貨號 | B222637 |
| Organism | Homo sapiens, human |
| Tissue | Melanoma, Pelvic Soft Tissue Metastasis |
| Cell Type | Melanocyte |
| Product Format | frozen |
| Morphology | Epithelial-like |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Melanoma, Stage IV; malignant |
| Age | 60 |
| Gender | Male |
| Ethnicity | Caucasian |
| Applications | Drug screening Development of targeted therapy Development of combination therapy Tumor vaccine development |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | Derived from a pelvic soft tissue recurrence. Established from digest of Right Pelvic Tumor Metastasis in PETI Medium. |
| Clinical Data | Primary Site: Left Foot (Plantar); Metastatic Site: Pelvic Soft Tissue |
| HLA Typing | A2,A31,B7,B51,Cw7?,Cw16?,DR4(DRB1*0404),[DR11(DRB1*1101) or DR13(DRB1*131401)],DR52(DRB3*0202),DR53(DRB4*0103) |
| Comments | NRAS Mutation: Q61L Q61R CDKN2A: wt BRAF: wt PET Scan + |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Volumes are for a T-75 flask; Adjust accordingly
|
| Cryopreservation | Fetal bovine serum, 90%; DMSO, 10% |
| Culture Conditions | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2 ), 5% |
| STR Profile | Amelogenin: X D5S818: 11 D13S317: 9,13 D7S820: 9 D16S539: 12 vWA: 17,19 TH01: 8,9.3 TPOX: 8,12 CSF1PO: 12 |
| Sterility Tests | Pass |
| Population Doubling Level (PDL) | unknown |
| Population Doubling Capacity | unknown |
| Name of Depositor | Craig L. Slingluff, Jr. M.D. |
| Passage History | Original deposit at one passage from the primary culture |
| Year of Origin | 2007 |
| References | Molhoek K, et al. Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res 21(4): 274–284, 2011. PubMed: 21654344 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
