The insert contains an internal SstI (SacI) site. Restriction digests of the clone give the following sizes (kb): PstI--4.0, 0.35; BamHI--5.0; EcoRI--5.0; HindIII--2.8, 2.3; SalI--5.0. This insert is free of repeats. Enzyme(s) not detecting polymorphism: PstI, RsaI, AccI. |