Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; BglI--2.4, 1.6, 1.05, 0.45; EcoRI--4.5, 1.15; PvuII-- 2.8, 2.5, 0.3; SacI--5.6. The insert contains the following restriction sites (nt from the 5' end): BglI--79, 389; SacI--709; PvuII--752. The insert is the 5' EcoRI fragment from clone lambda6, and contains the 5' untranslated region, the complete coding sequence, and the 3' untranslated region up to the naturally occurring EcoRI site at nt 1105. |