產(chǎn)品名稱 |
Influenza B virus |
商品貨號 |
B223956 |
Classification |
Orthomyxoviridae, Influenzavirus B |
Agent |
Influenza B virus |
Strain |
B/Taiwan/2/62 |
Applications |
Emerging infectious disease research Respiratory research Influenza Research |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Comments |
Derived by adaptation from allantoic product ATCC® VR-295™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-295™ and ATCC® VR-1735™ have not been compared for sequence or infectivity in chicken embryo culture and tissue culture. |
Effect on Host |
CPE, rounding and degeneration |
Recommended Host |
MDCK (ATCC® CCL-34) |
Growth Conditions |
Temperature: 33°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days |
Name of Depositor |
RQ Robinson, Centers for Disease Control and Prevention; ATCC® |
Special Collection |
ATCC |
Source |
Patient in Taiwan, 1962 |
References |
Green IJ, et al. The isolation and characterization of a new Influenza Type B virus on Taiwan. Am. J. Hyg. 79: 107-112, 1964. PubMed: 14114349
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