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Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.6; XhoI--4.6; PstI/XhoI--3.0, 1.6; EcoRI--4.6; PvuII--2.5, 2.1.
The insert contains the EcoRI site at nt 1570 of the published sequence.
Extends from the PstI site at nt 186 of the published sequence (5' untranslated region) through the complete coding sequence into the 3' untranslated region to the SacI site at about nt 1950.
The polypeptide is encoded from nt 13 to 1728 of the final published sequence.
Oriented such that transcription from the T7 promoter gives the sense strand.
Royer-Pokora B, et al. Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location. Nature 322: 32-38, 1986. PubMed: 2425263