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p659-2
p659-2
規(guī)格:
貨期:
編號:B224193
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p659-2
商品貨號 B224193
Designations p659-2
Species Homo sapiens, human
Depositors HG Zachau, W Pargent
Vector
Construct size (kb): 4.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 1.200000047683716
Tissue: placenta
Gene product: immunoglobulin kappa variable region [IGKV]
Alleles: Gd, Nd, Gd, Gd, Gd, Gp, Nd, Nd, Np, Np, Np, Gp, Gp, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Np, Gp, Np, Gp, Np, Gp, Np, Gp
Insert Size (kb) 1.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): SalI/SmaI--3.0, 1.2; PvuII--2.5, 1.7; PvuI--3.1, 1.1; XhoI/XbaI--3.0, 1.2; KpnI/EcoRI--3.0, 1.2.
Detects sequences from the Ap (between A29 and A30) and the Ad (between A13 and A14) regions.
Recloned from m659-2 which had been constructed by inserting a Sau3AI fragment into the BamHI site of M13mp10.
References

Lorenz W, et al. Physical map of the human immunoglobulin K locus and its implications for the mechanisms of VK-JK rearrangement. Nucleic Acids Res. 15: 9667-9676, 1987. PubMed: 3122179

Pargent W, et al. Polymorphisms and haplotypes in the human immunoglobulin kappa locus. Eur. J. Immunol. 21: 1829-1835, 1991. PubMed: 1678350

Hans G Zachau, personal communication

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