Transient expression plasmid for chloramphenicol acetyltransferase activity in cultured mammalian cells under the control of the vaccinia promoter.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Cochran MA, et al. Eukaryotic transient expression system dependent on transcription factors and regulatory DNA sequences of vaccinia virus. Proc. Natl. Acad. Sci. USA 82: 19-23, 1985. PubMed: 3855541