Restriction digests of the clone give the following sizes (kb): EcoRI--22.0, 13.0, 6.4, 5.0, 2.6. Verified to contain the correct insert sequence using the following oligonucleotides (5'-3'): CCATATGAATGCACCAAGTCGGAC and AAATGCAGGCCTGCTTACATTCC. The predicted product size is (bp): 119. The observed product size is (bp): 119. |