| 產(chǎn)品名稱 | NM2C5 |
|---|---|
| 商品貨號(hào) | B225120 |
| Organism | Homo sapiens, human |
| Cell Type | melanocyte, Melanoma |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | cancer |
| Age | 31 years |
| Gender | female |
| Ethnicity | Caucasian |
| Applications | These well characterized, tumorigenic human isogenic cell lines have dramatically opposite metastatic phenotypes and are ideal for metastatic studies. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The parental cell lines ATCC CRL-2918 (NM2C5) and ATCC CRL-2914 ?(M4A4) were derived from the human breast cancer cell line, MDA-MB-435. Note: Recent studies have generated questions about the origin of the parent cell line, MDA-MB-435. Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The NM2C5 GFP (ATCC CRL-2919) cell line was developed by the transduction of the GFP gene into NM2C5 (ATCC CRL-2918) cell line. |
| Clinical Data | 31 years
Caucasian
female |
| Antigen Expression | CD44; Homo sapiens, expressed |
| Receptor Expression | epidermal growth factor (EGF), expressed |
| Oncogene | c-myc; Ras; p53 |
| Comments | Note: Recent studies have generated questions about the origin of the parent cell line, MDA-MB-435. Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line.
M4A4 is highly metastatic in immuno-deprived mice, while NM2C5 is weakly or virtually non-metastatic. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line Ref |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | To avoid phenotypic drift it is recommended to make frozen aliquots of the cells and use each aliquot for only 10 passages.
Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:12 is recommended
Medium Renewal: 2 to 3 times a week
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation | Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC? Catalog No. 4-X.
|
| Culture Conditions | Temperature: 37°C
Atmosphere: 5% CO2 in air recommended |
| STR Profile | Amelogenin: X CSF1PO: 11 D13S317: 12 D16S539: 13 D5S818: 11,12 D7S820: 8,10 THO1: 6,7 TPOX: 8,11 vWA: 16,18 |
| Population Doubling Time | about 31 hours |
| Name of Depositor | D Tarin |
| Year of Origin | 1992 |
| References | Ross DT, et al. Systematic variation in gene expression patterns in human cancer cell lines. Nature Genetics 24: 227-235, 2000. PubMed: 10700174 Bao L, et al. Correlation of VLA-4 integrin expression with metastatic potential in various human tumour cell lines. Differentiation 52: 239-246, 1993. PubMed: 7683291 Urquidi V, et al. Contrasting expression of thrombospondin-1 and osteopontin correlates with absence or presence of metastatic phenotype in an isogenic model of spontaneous human breast cancer metastasis. Clin. Cancer Res. 8: 61-74, 2003. PubMed: 11801541 Rae JM, et al. Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties. Clin. Exp. Metastasis 21: 543-552, 2004. PubMed: 15679052 Goodison S, et al. Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from nonmetastatic breast tumors as revealed by labeling with green fluorescent protein. Clin. Cancer Res. 9: 3808-3814, 2003. PubMed: 14506175 Montel V, et al. Expression profiling of primary tumors and matched lymphatic and lung metastases in a xenogeneic breast cancer model. Am. J. Pathol. 166: 1565-1579, 2005. PubMed: 15855655 Suzuki M, et al. Dormant cancer cells retrieved from metastasis-free organs regain tumorigenic and metastatic potency. Am. J. Pathol. 169: 673-681, 2006. PubMed: 16877365 Montel V, et al. Tumor-stromal interactions reciprocally modulate gene expression patterns during carcinogenesis and metastasis. Int. J. Cancer 119: 251-263, 2006. PubMed: 16482564 Goodison S, et al. Molecular cytogenetic analysis of a human breast metastasis model: identification of phenotype-specific chromosomal rearrangements. Cancer Genet. Cytogenet. 156: 37-48, 2005. PubMed: 15588854 Hayashi K, et al. Differential effects of retinoic acid on the growth of isogenic metastatic and non-metastatic breast cancer cell lines and their association with distinct expression of retinoic acid receptor beta isoforms 2 and 4. Int. J. Oncol. 22: 623-629, 2003. PubMed: 12579317 Tarin DTumor metastasisIn: Tarin DOxford Textbook of PathologyOxford, United KingdomOxford University Press607-663, 1992 Sellappan S, et al. Lineage infidelity of MDA-MB-435 cells: expression of melanocyte proteins in a breast cancer cell line. Cancer Res. 64: 3479-3485, 2004. PubMed: 15150101. Ellison G, et al. Further evidence to support the melanocytic origin of MDA-MB-435. Mol. Pathol. 55: 294-299, 2002. PubMed: 12354931. Rae JM, et al., MDA-MB-435 cells are derived from M14 Melanoma cells - a loss for breast cancer, but a boon for melanoma research. Breast Cancer Res. Treat. 104:13-19, 2007. PubMed: 17004106. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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Rae JM, et al. Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties. Clin. Exp. Metastasis 21: 543-552, 2004. PubMed: 15679052