Insert contains 4 MspI, 1 HhaI, 6 AvaI, 1 SmaI sites. Restriction digests of the clone give the following sizes (kb): SalI--uncut; HindIII--4.3; PstI--4.3; BamHI--4.3; BamHI/PstI--2.7; 1.6. This probe detects genomic sequences showing varying levels of methylation. Failed to hybridize with mouse DNA. Derived from first intron. |