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pUH84
pUH84
規(guī)格:
貨期:
編號:B225359
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pUH84
商品貨號 B225359
Designations pUH84
Depositors B Henrich
Biosafety Level 1
Vector Information
Size (kb): 3.19
Vector: pUH84 (plasmid)
Marker(s):ampR
Construct size (kb): 3.19
Features: insert detection: phiX174lysis
marker(s): ampR
replicon: pMB1
repressor gene: lacIq
Applications
vector permitting positive selection for inserts
Comments
The plasmid may be lost from transformants if stored as stabs or at -20C. For propagation and maintenance use strains with repressor-overproducing alleles of lacI; for cloning use strains that do not overproduce the lac repressor.
A positive selection plasmid vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Henrich B, Plapp R. Use of the lysis gene of bacteriophage phiX174 for the construction of a positive selection vector. Gene 42: 345-349, 1986. PubMed: 2942443

Henrich B, et al. Lysis of Escherichia coli by induction of cloned phiX174 genes. Mol. Gen. Genet. 185: 493-497, 1982. PubMed: 6285147

Shipped freeze-dried
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