產(chǎn)品名稱 |
Canine adenovirus 2 |
商品貨號 |
B225404 |
Classification |
Adenoviridae, Mastadenovirus, Canine adenovirus A |
Deposited As |
Canine adenovirus |
Agent |
Canine adenovirus 2 |
Strain |
Toronto A 26/61 |
Applications |
Respiratory research |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Isolation |
Throat swab from a dog with laryngotracheitis |
Product Format |
frozen 1 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Effect on Host |
CPE, cell rounding and sloughing |
Recommended Host |
MDCK (ATCC CCL-34) |
Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-8 days |
Name of Depositor |
LN Binn, Walter Reed Army Institute of Research, Washington, DC |
Source |
Throat swab from a dog with laryngotracheitis |
References |
Ditchfield J, et al. Association of a canine adenovirus (Toronto A 26/61) with an outbreak of laryngotracheitis (“Kennel Cough”): A Preliminary Report. Can. Vet. J. 3: 238-247.1962, PubMed: 17421510
Kapsenberg JG. Relationship of infectious canine hepatitis virus to human adenovirus. Proc. Soc. Exp. Biol. Med. 101: 611-614, 1959. PubMed: 14404448
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