Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 6.71 (vector); 6.05, 4.20, 4.19, 3.62, 2.99, 2.90, 2.33, 2.15, 2.06, 1.69, 1.53, 1.39, 1.05, 1.05, 0.80, 0.73, 0.73. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 313890-352000 of the sequence of chromosome VIII. |
References |
Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229
Linda Riles, personal communication
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