產(chǎn)品名稱 |
pC63 |
商品貨號(hào) |
B225963 |
Designations |
pC63 |
Species |
Homo sapiens, human |
Depositors |
M Litt |
Applications |
Enzyme(s) not detecting polymorphism: TaqI, RsaI, HinfI, EcoRI, HindIII, PstI, PvuII, BglII, BamHI, SacI, BstEII (tested against 6 unrelated Caucasians). |
Vector |
Construct size (kb): 4.400000095367432 |
Insert |
DNA: genomic Insert lengths(kb): 1.700000047683716 Tissue: DNA from mouse/human PCTBA1.8 hybrid cell line Gene product: DNA Segment, single copy [D17S21] Alleles: A1, A2 |
Insert Size (kb) |
1.700 |
Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information |
Distributed: freeze-dried |
Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.7; SalI--4.4; BamHI--4.4; HindIII--4.4; PstI--4.4. Codominant segregation of the MspI polymorphism has been demonstrated in Utah families K1329C and K1333 with a total of 15 children. Enzyme(s) not detecting polymorphism: TaqI, RsaI, HinfI, EcoRI, HindIII, PstI, PvuII, BglII, BamHI, SacI, BstEII (tested against 6 unrelated Caucasians). |
References |
Kondoleon S, et al. An anonymous single-copy clone, pC63, from chromosome 17q23-qter identifies a frequent RFLP [HGM9 No. D17S21]. Nucleic Acids Res. 15: 9096, 1987. PubMed: 2891104
Vissing H, et al. Progress towards construction of a total restriction fragment map of a human chromosome. Nucleic Acids Res. 15: 1363-1374, 1987. PubMed: 3029713
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