Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Preceptrol®
no
Genotype
thiD thiL
Type Strain
no
Comments
Mutagenesis of ATCC 49562 with N-methyl-N'-nitro-N-nitrosoguanidine. Biosynthesis of thiamine pyrophosphate
Growth inhibited by thiamine or thiamine monophosphate
Medium
ATCC® Medium 1746: LB medium (ATCC medium 1065) with 0.1 mM thiamine pyrophosphage
Growth Conditions
Temperature: 37.0°C
Name of Depositor
BJ Bachmann
Chain of Custody
ATCC <<--BJ Bachmann<<--H. Nakayama
References
Nakayama H, Hayashi R. Inhibition of thiamine pyrophosphate utilization by thiamine or its monophosphate in Escherichia coli. J. Bacteriol. 118: 32-40, 1974. PubMed: 4595201
Nakayama H, Hayashi R. Biosynthesis of thiamine pyrophosphate in Escherichia coli. J. Bacteriol. 109: 936-938, 1972. PubMed: 4550824
Nakayama H, Hayashi R. Biosynthetic pathway of thiamine pyrophosphate: a special reference to the thiamine monophosphate-requiring mutant and the thiamine pyrophosphate-requiring mutant of Escherichia coli. J. Bacteriol. 112: 1118-1126, 1972. PubMed: 4565529