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Size (kb): 4.320

Vector: pFN476 (plasmid)

Promoters: Promoter lac

Construction: pGEM3Z, pKA101-6

Marker(s):ampR

Construct size (kb): 4.320

Features: insert detection: lacZ'
marker(s): ampR
other: par
promoter: lac
promoter for expression: T7
promoter for in vitro transcription: SP6
replicon: pSC101
replicon: rep (pSC101)
MCS: pGEM-3Z; HindIII...EcoRI
restriction site: BamHI
coding sequence: tnpR

expression vector T7 promoter

vector permitting RNA synthesis in vitro

vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase

vector with low copy number

Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; HindIII--4.2; PstI--3.1, 1.1.

Low copy number vector to express transcripts in vivo from the T7 promoter and to minimize toxic gene effects.

Plasmid contains the pSC101 replicon and is maintained at 1-5 copies per cell.

Low copy number minimizes the effect of uninduced expression of T7 RNA polymerase, reducing transcription of potentially toxic inserts.

T7 RNA polymerase can also be provided by infection of F' hosts with M13mGP1-2 (ATCC 40303).

Temperature: 37.0°C