Quantitative Synthetic Human papillomavirus 18 DNA
商品貨號
B227105
Agent
Quantitative Synthetic Human papillomavirus 18 DNA
Common Name
HPV-18
Applications
ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen Specification range: 1 x 105 to 1 x 106 copies/µL 100 µL per vial with Biomatrica DNAstable
Storage Conditions
-20°C or colder
Intended Use
The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification,
or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.
Comments
Manufactured under ISO 13485 guidance
Preparation includes full length genome of human papillomavirus type 18 derived from a plasmid clone (ATCC® 45152D).
The following primers and probe can be used with this nucleic acid preparation RefSeaman WT, et al. Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR. Virol. J. 7: 194, 2010. PubMed: 20723234:
Forward primer: GCATAATCAATTATTTGTTACTGTGGTAGATACCACT
Reverse primer: GCTATACTGCTTAAATTTGGTAGCATCATATTGC
Probe: FAM-AACAATATGTGCTTCTACACAGTCTCCTGT-BHQ
Special Collection
DNA
References
Seaman WT, et al. Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR. Virol. J. 7: 194, 2010. PubMed: 20723234
Cross References
Nucleotide (GenBank) :
AY262282
Human papillomavirus type 18 complete sequence